Dr Paul Dyer

Senior Lecturer in Biomedical Science

Faculty:Faculty of Science & Technology

Department:Biomedical and Forensic Science

Location: Cambridge

Areas of Expertise: Haematology , Cell and Molecular Biology

Courses taught: Biomedical Science, Biomedical Science

Paul is a Senior Lecturer in Biomedical Science and HCPC registered Biomedical Scientist. His research aim to develop the application of biological nanoparticles and exosomes as novel biomarkers and drug delivery agents.

paul.dyer@anglia.ac.uk

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ORCiD 0000-0003-2348-7870

Background

Paul Joined the Department of Biomedical and Forensic Science as a Senior Lecturer in November 2017. Prior to this, he was a Principal Lecturer in the Department of Life and Sport Science at the University of Greenwich.

Paul has a strong background in Biomedical Science education and training having worked in both the NHS as a state registered Biomedical Scientist and HE sector teaching at both undergraduate and postgraduate levels.

Upon completing his PhD in 2014, Paul has been expanding upon his research in:

  • mimetic drug delivery platforms with Dr Simon Richardson (University of Greenwich)
  • investigating the application of biophysical techniques to explore the biology associated with protein pores and channels in biologically relevant lipid bilayers, in collaboration with Dr Robert Barker (University of Kent), Prof. Alistair Mathie (University of Kent), Dr Emma Veale (University of Kent) and Dr Oliver Castell (Cardiff University)
  • determining the role of the host cell immune response in the pathogenesis and spread of Leishmania spp., in collaboration with Dr Giulia Getti (University of Greenwich).

He is currently engaged in developing his research group in the Applied Exosomes (ApEx) Research Laboratory, which aims to further enhance our understanding of exosomes and microvesicles, realising their potential as novel diagnostic biomarkers and personalised medicines.

Research interests

  • The application of exosomes as novel biomarkers and drug delivery agents.
  • The structural constraints of protein translocation though bacterial toxin pores.
  • The role of host immunological response in the pathogenicity and spread of Leishmania spp.
  • Investigation of protein pores and channels in lipid membranes.
  • Application of biophysical techniques to examine proteins in biologically relevant lipid bilayers, lipid nanodiscs and exosomes.

Areas of research supervision

  • PhD supervisor on a project to characterise the dynamics of the Bacillus anthracis Protective Antigen (PA) pore for biomedical applications (2016).
  • PhD supervisor on a project to determine the intracellular and intercellular spread of Leishmania parasites, focussing on L. mexicana and L. aethiopica. (2016).
  • PhD supervisor on a project investigating the translocation of proteins through the Bacillus anthracis Protective Antigen (PA) translocase – Completed 2017

Teaching

BSc (Hons) Biomedical Science
Module Leader for Diagnostic Techniques in Pathology
Module Leader for Preparation for Research

MSc Biomedical Science (Distance Learning)
Module Leader for Cellular Haematology
Module Leader for Haemostasis and Blood Transfusion

Qualifications

PhD Cell Biology and Drug Delivery - University of Greenwich (2014)
MSc Haematology (Blood Transfusion) - University of Westminster (2001)
BSc (Hons) Biomedical Science - University of Bradford (1997)

Memberships, editorial boards

  • HCPC Registered Biomedical Scientist (BS41478)
  • Fellow of the Institute of Biomedical Science (FIBMS)
  • Associate Member of the Royal Society of Chemistry (AMRSC)
  • Member of the Biochemical Society

Research grants, consultancy, knowledge exchange

Science and Technology Facilities Council (STFC) (2017). Investigation of the translocation potential of therapeutic macromolecules into cell derived exosomes: RB1710290. 4 Days beam time at ISIS/RAL – In-kind value ~£72, 000

Institute Langevin –Laue (ILL), Grenoble (2016). Determination of the presence of deuterated lethal factor in cell derived exosomes using SANS and neutron reflectometry: 8-02-786. 4 Days total beam time requested – In-kind value £60, 000

University of Greenwich, Competitive REF allocation (2016). Characterisation of two-pore-domain potassium (K2P) channels in direct association with putative therapeutic agents, using novel nanodisc technology. £18, 000

Medway School of Pharmacy, University of Kent PhD Studentship (2016). Characterisation of two-pore-domain potassium (K2P) channels in direct association with putative therapeutic agents, using novel nanodisc technology. £45, 000

Science and Technology Facilities Council (STFC) (2016). Investigation of the solution structure and pore dynamics of Bacillus anthracis protective antigen stabilised in lipid nanodiscs: RB1610353. 4 Days beam time at ISIS/RAL – In-kind value £72, 000

Institute Langevin –Laue (ILL), Grenoble (2015). Investigation of PA pore translocation through lipid nanodiscs using SANS and neutron reflectometry: 8-03-848. 3 Days beam time at ILL – In-kind value £45, 000

University of Greenwich, Department Investment Fund (2014). EVOS FLoid Cell Imaging Station to enhance student learning, experience and outreach - £25, 000

University of Greenwich, Department Investment Fund (2014). Investigation of protein production, purification and characterisation by HSDSC, Kinetic CD, SANS and Neutron Reflectometry - £25, 000

Greenwich Research and Enterprise: Research and Enterprise Investment Programme (2008).  Characterisation of a novel RNAi delivery system. £14, 857

Selected recent publications

Rai, R., Dyer, P., Richardson, S., Harbige, L., & Getti, G., 2017. Apoptotic induction induces Leishmania aethiopica and L. mexicana spreading in terminally differentiated THP-1 cells. Parasitology, 144(14), pp1912-1921. doi:10.1017/S0031182017001366

Shorter S. A., Pettit M. W., Dyer P. D. R., Coakley-Youngs J. E., Gorringe-Pattrick M. A. M., El-Daher S. and Richardson S. C. W., 2017. Green Fluorescent Protein (GFP): is seeing believing and is that enough? Journal of Drug Targeting, 25(9-10), pp809-817 doi:10.1080/1061186X.2017.1358725

Paul D. R. Dyer, Arun  K Kotha, Alexander S Gollings, Susan A Shorter, Thomas R Shepherd, Marie W Pettit, Bruce  D. Alexander, Giulia T Getti, Samer El-Daher, Les  Baillie, Simon C. W. Richardson, 2016. An in vitro evaluation of epigallocatechin gallate (eGCG) as a biocompatible inhibitor of ricin toxin. Biochimica et Biophysica Acta (BBA), 1860(7), pp1541-50 doi:10.1016/j.bbagen.2016.03.024

Shorter S.A., Gollings A.S., Gorringe-Pattrick M.A.M., Coakley J.E., Dyer P.D.R. and Richardson S.C.W., 2016. The potential of toxin-based drug delivery systems for enhanced nucleic acid therapeutic delivery. Expert Opinion on Drug Delivery, 14(5), pp685-696, doi:10.1080/17425247.2016.1227781

Paul D.R. Dyer, Thomas R. Shepherd, Alexander S. Gollings, Susan A. Shorter, Monique A.M. Gorringe-Pattrick, Chun-Kit Tang, Beatrice N. Cattoz, Les Baillie, Peter C. Griffiths, Simon C.W. Richardson, 2015. Disarmed anthrax toxin delivers antisense oligonucleotides and siRNA with high efficiency and low toxicity. Journal of Controlled Release, 220(Part A), pp316-328. doi:10.1016/j.jconrel.2015.10.054     

Marie W. Pettit, Paul D.R. Dyer, John C Mitchell, Peter C Griffiths, Bruce Alexander, Beatrice Cattoz, Richard K Heenan, Stephen M King, Ralf Schweins, Frank Pullen, Stephen R Wicks, and Simon C W Richardson, 2014. Construction and physiochemical characterisation of a multi-composite, potential oral vaccine delivery system (VDS). International Journal of Pharmaceutics, 468(1-2), pp264-271. doi:10.1016/j.ijpharm.2014.03.046

Paul D. R. Dyer, Arun K. Kotha, Marie W.Pettit, Simon C. W. Richardson, 2013. Imaging Select Mammalian Organelles Using Fluorescent Microscopy: Application to Drug Delivery. In Cellular and subcellular nanotechnology: methods and protocols; Methods in Molecular Biology, Humana Press ISBN: 978-1-62703-336-7. doi:10.1007/978-1-62703-336-7_19

P.C. Griffiths, N. Mauro, D.M. Murphy, E. Carter, S.C. Richardson, P. Dyer and P. Ferruti, 2012. Self-assembled PAA-based nanoparticles as potential gene and protein delivery systems. Macromolecular Bioscience, 13(5), pp641–649. doi:10.1002/mabi.201200462

Dyer P.D.R. and Richardson S.C.W.*, 2011. Delivery of biologics to select organelles - the role of biologically active polymers. Expert Opinion on Drug Delivery, 8(4), pp403-7. doi:10.1517/17425247.2011.558080

Recent presentations and conferences

15th Medical Biodefense Conference. Munich, Germany, 28 April 2016. Invited speaker. An evaluation of epigallocatechin gallate (eGCG) as a biocompatible inhibitor of ricin and Clostridium difficile toxins. Dyer, P., Shorter, S., Richardson, S.C.W., and Baillie, L.

Media experience

http://www.stfc.ac.uk/news/anthrax-toxin-a-new-tool-in-the-fight-against-cancer/