Dr Nicholas Pugh

Senior Lecturer

Faculty:Faculty of Science & Technology

Department:Biomedical and Forensic Science

Location: Cambridge

Areas of Expertise: Life sciences

Nick's research interests concern the signalling mechanisms used by platelets during pathophysiological thrombus formation. He's particularly interested in the role of ion channels and transporters in platelet function.

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nicholas.pugh@anglia.ac.uk

Background

Having gained his PhD at Imperial College, London in 2003, Nick went on to work as a postdoctoral research scientist at the Babraham Institute (2003-2005) and at the Department of Biochemistry, University of Cambridge (2005-2013).

Nick's current research into platelet activation is supported by a British Heart Foundation project grant.

Research interests

  • The role of ion channels and transporters in transmembrane signalling in platelets
  • Quantification of platelet behaviour during thrombus formation in flowing blood
  • Effect of cations on coagulation
  • Effect of fatty acid accumulation on insulin signalling

Teaching

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BSc (Hons) Biomedical Science, MSc Biomedical Science

Qualifications

  • PhD, University of London
  • BSc (Hons), University of Southampton

Memberships, editorial boards

  • Member, Biochemical Society

Selected recent publications

Pugh, N., Bihan, D., Perry, D.J. and Farndale, R.W., 2015. Dynamic analysis of platelet deposition to resolve platelet adhesion receptor activity in whole blood at arterial shear rate. Platelets, 26(3), pp.216-19.

de Witt, S.M., Swieringa, F., Cavill, R., Lamers, M.M., van Kruchten, R., Mastenbroek, T., Baaten, C., Coort, S., Pugh, N., Schulz, A., Scharrer, I., Jurk, K., Zieger, B., Clemetson, K.J., Farndale, R.W., Heemskerk, J.W. and Cosemans, J.M., 2014. Identification of platelet function defects by multi-parameter assessment of thrombus formation. Nat Commun, 16(5), p.4257.

Sage, S.O., Pugh, N., Farndale, R.W. and Harper, A.G.S., 2013. Pericellular Ca2+ recycling potentiates thrombin-evoked Ca2+ signals in human platelets. Physiol Rep, 1(5):e00085.

Jung, S.M., Moroi, M., Soejima, K.Nakagaki, T., Miura, y., Berndt, M.C., Gardiner, E.E., Howes, J-M., Pugh, N, Bihan, D., Watson, S.P. and Farndale, R.W., 2012. Constitutive dimerization of glycoprotein VI (GpVI) in resting platelets is essential for binding to collagen and activation through this receptor in flowing blood. J Biol Chem, 24, 287(35):30000-13.

Slatter, D.A., Bihan, D., Jarvis, G.E., Stone, R., Pugh, N., Giddu, S. and Farndale R.W., 2012. The properties conferred upon triple-helical collagen-mimetic peptides by the presence of cysteine residues. Peptides, 36(1), pp.86-93.

Hamaia, S.W., Pugh, N., Raynal, N., Némoz, B., Stone, R., Gullberg, D., Bihan, D. and Farndale, R.W., 2012. Mapping of Potent and Specific Binding Motifs, GLOGEN and GVOGEA, for Integrin α1β1 Using Collagen Toolkits II and III. J Biol Chem, 27, 287(31):26019-28.

Grover, C.N., Gwynne, J.H., Pugh, N., Hamaia, S., Farndale, R.W., Best, S.M. and Cameron, R.E., 2012. Crosslinking and composition influence the surface properties, mechanical stiffness and cell reactivity of collagen-based films. Acta Biomater, 8(8):3080-90.

Jarvis, G.E., Bihan, D., Hamaia, S., Pugh, N., Ghevaert, C.J., Pearce, A.C., Hughes, C.E., Watson, S.P., Ware, J., Rudd, C.E. and Farndale, R.W., 2012. A Role for ADAP in Collagen-Induced Platelet Activation Mediated via Integrin α2β1. J. Thromb Haemost, 10(2), pp.268-77.

Pugh, N., Jarvis, G.E., Koch, A., Davies, B.G., Sakariassen, K.S. and Farndale, R.W., 2012. The impact of Factor Xa inhibition on axial dependent arterial thrombus formation triggered by a tissue factor rich surface. J Thromb Thrombolysis, 33(1), pp.6-15.

Barrow, A.D., Raynal, N., Andersen, T.L., Slatter, D., Bihan, D., Pugh, N., Cella, M., Kim, T., Rho, J., Negishi-Koga, T., Delaisse, J-M., Takayanagi, H., Lorenzo, J., Colonna, M., Farndale, R.W., Choi, Y. and Trowsdale J., 2011. OSCAR is a collagen receptor that co-stimulates osteoclastogenesis. J Clin Invest, 121(9):3505-16.

Sage, S.O., Mason, M.J., Pugh, N., Farndale, R.W. and Harper, A.G.S., 2011. Monitoring the intracellular store Ca2+ concentration in agonist-stimulated, intact human platelets using Fluo-5N. J Thromb Haemost, 9(3), pp.540-51.

Pugh, N., Simpson A.M.C, Smethurst P.A., de Groot P.G., Raynal N. and Farndale R.W., 2010. Synergism between platelet collagen receptors defined using receptor-specific collagen-mimetic peptide substrata in flowing blood. Blood, 115(24), pp.5069-79.

Saunders A., Webb L.M., Janas M.L., Hutchings A., Pascall J., Carter C., Pugh N., Morgan G., Turner M. and Butcher G.W., 2010. Putative GTPase GIMAP1 is critical for the development of mature B and T lymphocytes. Blood, 115(16), pp.3249-57.

Leo J.C., Elovaara H., Bihan D., Pugh N., Kilpinen S.K., Raynal N., Skurnik M., Farndale R.W. and Goldman A., 2010. First analysis of a bacterial collagen-binding protein with collagen Toolkits: promiscuous binding of YadA to collagens may explain how YadA interferes with host processes. Infect Immun, 78(7), pp.3226-36.

Walker, A., Pugh, N., Garner, S.F., Stephens, J., Maddox, B., Ouwehand, W.H., Farndale, R.W. and Steward, M. on behalf of the Bloodomics Consortium, 2009. Single Domain Antibodies against the Collagen Signalling Receptor Glycoprotein VI are Inhibitors of Collagen Induced Thrombus Formation. Platelets, 20(4), pp.268-76.

Farndale, R.W., Lisman, T., Bihan, D., Hamaia, S., Smerling, C.S., Pugh, N., Konitsiotis, A., Leitinger, B., de Groot, P.G., Jarvis, G.E. and Raynal, N., 2008. Cell-collagen interactions: the use of peptide Toolkits to investigate collagen-receptor interactions. Biochem Soc Trans, 36(Pt 2), pp.241-50.

Dion, C., Carter, C., Hepburn, L., Coadwell, W.J., Morgan, G., Graham, M., Pugh, N., Anderson, G., Butcher, G.W. and Miller, J.R., 2005. Expression of the Ian family of putative GTPases during T cell development and description of an Ian with three sets of GTP/GDP-binding motifs. Int. Immunol, 17(9), pp.1257-68.

Pugh, N., Mead-Savery, F.C., Coadwell, W.J., Rossi, D., Sorrentino, V. and Williams, A.J., 2005. Probing luminal negative charge in the type 3 ryanodine receptor. Biochem. Biophys. Res. Commun, 337(4), pp.1072-79.